Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma.

نویسندگان

  • K L Collart
  • R Aurigemma
  • R E Smith
  • S Kawai
  • H L Robinson
چکیده

To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken 6561) contained novel fos-related EcoRI and KpnI fragments which hybridized to both v-fos and viral probes. Oncogenicity tests using filtered 6561 tumor cell homogenates did not reveal a tumor-inducing transduction of c-fos. We conclude that c-fos is only an occasional target for proviral insertions or new transductions in avian leukosis virus-induced nephroblastoma. The results also identify a polymorphism in c-fos in K28 chickens and demonstrate that unintegrated viral DNA is not a general characteristic of avian leukosis virus-induced nephroblastoma.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The oncogenic effects of nontransforming viruses from avian myeloblastosis virus.

Standard avian myeloblastosis virus (AMV), BAI strain A, causes myeloblastosis, osteopetrosis, lymphoid leukosis, and nephroblastoma in chickens. In this investigation, several leu kosis viruses of subgroups A and B were isolated (rom standard AMV and characterized. A subgroup A virus derived from an AMV-induced lymphoma of a Japanese quail was isolated, and it induced a high incidence of osteo...

متن کامل

Metastatic cutaneous neuro- myofibroblastic sarcoma induced by avian leukosis virus subgroup J in a rooster (Gallus gallus domesticus)

An adult native cock (Gallus gallus domesticus) referred to the aviary clinic with multiple different sizes of round dermal nodules. The bird died few days later, and was then submitted for further evaluation. Macroscopic and microscopic examinations as well as a PCR test were done to identify type and cause of the tumor. In histopathological assessment of biopsy specimen, it consisted of inter...

متن کامل

Serologic profile of avian leukosis virus subgroup-J, Mycoplasma gallisepticum and Mycoplasma synoviae in broiler grandparent flocks of Iran.

  Avian Leukosis Virus Subgroup-J (ALV-J). Mycoplasma gallisepticum (MG) and MycopJasma synviae (MS) are important pathogens in chickens that cause severe economical losses in poultry industry throughout the world. Seven broiler grandparent flocks of Iran (six broiler strains) were sampled randomly at the ages of 8-63 wk (100 samples from each flock) for antibody detection to ALV-J, MG, and MS ...

متن کامل

Detection of avian leukosis virus (ALV) in albumen of Shiraz commercial and local layer flocks using ELISA and RT-PCR

Avian leukosis viruses (ALVS) cause different types of tumours in poultry and can affect the health and egg production of the birds. To investigate the presence of the virus in chicken layer flocks in Shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of virology

دوره 64 7  شماره 

صفحات  -

تاریخ انتشار 1990